Journal: Toxicological sciences : an official journal of the Society of Toxicology
Article Title: Contributions of aryl hydrocarbon receptor genetic variants to the risk of glioma and PAH-DNA adducts.
doi: 10.1093/toxsci/kfs158
Figure Lengend Snippet: Fig. 1. The distribution and staining intensity of PAH-DNA adducts in the TMA analysis using IHC and the association with the glioma grade. (A) Repre- sentative immunoperoxidase staining using 5D11 monoclonal antibody for PAH-DNA adducts of paraffin-embedded TMA; the adducts were detected in normal brain and gliomas of grades I–IV, and the staining intensity was significantly associated with the glioma grade. (B) The PAH-DNA adducts staining intensity index increased with increasing glioma grades (with a mean staining index value of 31.363 for normal, 66.146 for Grade I, 85.647 for Grade II, 156.597 for Grade III, and 190.714 for Grade IV). The Avg. indicates the mean values.
Article Snippet: PAH-DNA adducts of TMA sections were reacted with a benzo(a)pyrene diol epoxide (BPDE)-DNA (5D11) monoclonal antibody (sc-52625; Santa Cruz, 1:100 dilution) and a horseradish peroxidase–conjugated secondary antibody.
Techniques: Staining, Immunoperoxidase Staining